Asthma Allergy Immunology

Asthma Allergy Immunology

2009, Vol 7, Num, 2     (Pages: 100-105)

Different IgE reactivity profiles in the patients who sensitized to Betula pendula Roth (syn = B. verrucosa) pollen

Şenol ALAN 1, Özlem YILDIRIM 2, Nur Münevver PINAR 2, Derya SEÇİL 3, Tamer KEÇELİ 4, Talip ÇETER 5, Zeynep MISIRLIGİL 3,

1 Zonguldak Karaelmas Üniversitesi Fen-Edebiyat Fakültesi, Biyoloji Bölümü, Zonguldak, Türkiye
2 Ankara Üniversitesi Fen Fakültesi, Biyoloji Bölümü, Ankara, Türkiye
3 Ankara Üniversitesi Tıp Fakültesi, Göğüs Hastalıkları Anabilim Dalı, Allerjik Hastalıklar Bilim Dalı, Ankara, Türkiye
4 Çankırı Karatekin Üniversitesi Fen-Edebiyat Fakültesi, Biyoloji Bölümü, Çankırı, Türkiye
5 Kastamonu Üniversitesi Fen-Edebiyat Fakültesi, Biyoloji Bölümü, Kastamonu, Türkiye

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Objective: IgE reactivity has been investigated in the patients who sensitized to Betula pendula Roth (syn= B. verrucosa) pollen that distributed widely in nature habitat and also in park and gardens in Turkey.

Materials and Methods: Protein profile of pollen was studied by SDS-PAGE and transferred to polyvinylidenfluorid (PVDF) membrane. IgE reactivity profiles have been assessed via 10 patients who sensitized to pollen.

Results: It has been found that 86% (n= 6) show a positive reaction to 17 kDa (Bet v 1), 29% (n= 2) of sensitized seven patients has show a positive reaction to 15 kDa (Bet v 2). Only one patient show a reaction to protein which has 14.5 kDa molecular weight.

Conclusion: Our preliminary results have shown that the most common IgE reactivitiy was against Bet v 1 in Betula pollen allergy in our country similar to many European countries.

Keywords : Betula pendula, pollen, immunoglobulin E, Bet v 1 allergen, Bet v 2 allergen